Study 3: Minocycline Decreases Microglia Activation
Primary Purpose
Hypertension
Status
Not yet recruiting
Phase
Phase 2
Locations
United States
Study Type
Interventional
Intervention
Minocycline
Sponsored by

About this trial
This is an interventional treatment trial for Hypertension focused on measuring Resistent Hypertension
Eligibility Criteria
Inclusion Criteria:
- Subjects participating in IRB approved protocol #102-2013 will be eligible to participate.
- (For Study 3 Participants only) Willing to travel to Montreal, Canada for specialized imaging of the participant's brain using magnetic resonance imaging (MRI), positron emission tomography (PET) scanning, Autonomic Nervous System Testing and blood drawing- if participant qualifies
Exclusion criteria for control and resistant hypertensive subjects include:
- currently pregnant or have been pregnant in the last 6 months;
- antibiotic treatment within 2 months of study enrollment;
- currently taking a medication (e.g., antibiotic, anti-inflammatory agents, glucocorticoids or other immune modulating medications);
- unwilling to discontinue vitamin or supplements, including probiotics, potentially affecting gut microbiota (vitamins/supplements and medications that possibly affect the gut microbiota should be discontinued for at least 2wks prior to stool collection);
- history of intestinal surgery, inflammatory bowel disease, celiac disease, lactose intolerance, chronic pancreatitis or other malabsorption disorder.
Sites / Locations
- UF Health Cardiovascular Clinic
Arms of the Study
Arm 1
Arm Type
Other
Arm Label
Minocycline
Arm Description
Subjects will receive the dose of Minocycline determined to best lower BP and will undergo baseline and week 12-24 follow-up MRI and PET scans for changes in the paraventricular nucleus.
Outcomes
Primary Outcome Measures
MRI changes in the paraventricular nucleus
MRI changes in the paraventricular nucleus from baseline to 12 weeks. Increased binding in the autonomic brain regions (paraventricular nucleus)
MRI changes in the paraventricular nucleus
MRI changes in the paraventricular nucleus from baseline to 24 weeks. Increased binding in the autonomic brain regions (paraventricular nucleus)
PET changes in the paraventricular nucleus
PET changes in the paraventricular nucleus from baseline to 12 weeks. Increased binding in the autonomic brain regions (paraventricular nucleus)
PET changes in the paraventricular nucleus
PET changes in the paraventricular nucleus from baseline to 24 weeks. Increased binding in the autonomic brain regions (paraventricular nucleus)
Secondary Outcome Measures
Full Information
NCT ID
NCT02213575
First Posted
August 7, 2014
Last Updated
May 8, 2023
Sponsor
University of Florida
Collaborators
National Heart, Lung, and Blood Institute (NHLBI)
1. Study Identification
Unique Protocol Identification Number
NCT02213575
Brief Title
Study 3: Minocycline Decreases Microglia Activation
Official Title
Effect of Minocycline Treatment on Drug-Resistant Hypertensive Patients
Study Type
Interventional
2. Study Status
Record Verification Date
May 2023
Overall Recruitment Status
Not yet recruiting
Study Start Date
April 15, 2024 (Anticipated)
Primary Completion Date
April 15, 2025 (Anticipated)
Study Completion Date
October 15, 2025 (Anticipated)
3. Sponsor/Collaborators
Responsible Party, by Official Title
Sponsor
Name of the Sponsor
University of Florida
Collaborators
National Heart, Lung, and Blood Institute (NHLBI)
4. Oversight
Studies a U.S. FDA-regulated Drug Product
Yes
Studies a U.S. FDA-regulated Device Product
No
Product Manufactured in and Exported from the U.S.
Yes
Data Monitoring Committee
No
5. Study Description
Brief Summary
This study is a mechanistic study that will enroll 9 subjects who are participating in NCT02133885 (which is designed to evaluate minocycline to test the hypothesis that minocycline treatment would produce antihypertensive effects in drug-resistant neurogenic hypertensive individuals) to test whether the antihypertensive effect of minocycline is associated with a decrease in activated microglia in central nervous system autonomic regions as evidenced by changes in PET and MRI imaging.
Detailed Description
This study will recruit 9 subjects from NCT02133885 who will agree to undergo additional autonomic testing and imaging studies at baseline and after 3-6 months of study treatment. Specialized imaging of the brain using magnetic resonance imaging (MRI) and positron emission tomography (PET) scanning will be conducted at the Montreal Neurological Institute, in Montreal Canada.
6. Conditions and Keywords
Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Hypertension
Keywords
Resistent Hypertension
7. Study Design
Primary Purpose
Treatment
Study Phase
Phase 2
Interventional Study Model
Single Group Assignment
Masking
None (Open Label)
Allocation
N/A
Enrollment
9 (Anticipated)
8. Arms, Groups, and Interventions
Arm Title
Minocycline
Arm Type
Other
Arm Description
Subjects will receive the dose of Minocycline determined to best lower BP and will undergo baseline and week 12-24 follow-up MRI and PET scans for changes in the paraventricular nucleus.
Intervention Type
Drug
Intervention Name(s)
Minocycline
Intervention Description
Subjects will receive the dose of Minocycline determined to best lower BP and will undergo baseline and week 12-24 follow-up MRI and PET scans for changes in the paraventricular nucleus.
Primary Outcome Measure Information:
Title
MRI changes in the paraventricular nucleus
Description
MRI changes in the paraventricular nucleus from baseline to 12 weeks. Increased binding in the autonomic brain regions (paraventricular nucleus)
Time Frame
Change from baseline to 12 weeks
Title
MRI changes in the paraventricular nucleus
Description
MRI changes in the paraventricular nucleus from baseline to 24 weeks. Increased binding in the autonomic brain regions (paraventricular nucleus)
Time Frame
Change in Baseline to 24 weeks
Title
PET changes in the paraventricular nucleus
Description
PET changes in the paraventricular nucleus from baseline to 12 weeks. Increased binding in the autonomic brain regions (paraventricular nucleus)
Time Frame
Change from baseline to 12 weeks
Title
PET changes in the paraventricular nucleus
Description
PET changes in the paraventricular nucleus from baseline to 24 weeks. Increased binding in the autonomic brain regions (paraventricular nucleus)
Time Frame
Change in Baseline to 24 weeks
10. Eligibility
Sex
All
Minimum Age & Unit of Time
18 Years
Maximum Age & Unit of Time
85 Years
Accepts Healthy Volunteers
No
Eligibility Criteria
Inclusion Criteria:
Subjects participating in IRB approved protocol #102-2013 will be eligible to participate.
(For Study 3 Participants only) Willing to travel to Montreal, Canada for specialized imaging of the participant's brain using magnetic resonance imaging (MRI), positron emission tomography (PET) scanning, Autonomic Nervous System Testing and blood drawing- if participant qualifies
Exclusion criteria for control and resistant hypertensive subjects include:
currently pregnant or have been pregnant in the last 6 months;
antibiotic treatment within 2 months of study enrollment;
currently taking a medication (e.g., antibiotic, anti-inflammatory agents, glucocorticoids or other immune modulating medications);
unwilling to discontinue vitamin or supplements, including probiotics, potentially affecting gut microbiota (vitamins/supplements and medications that possibly affect the gut microbiota should be discontinued for at least 2wks prior to stool collection);
history of intestinal surgery, inflammatory bowel disease, celiac disease, lactose intolerance, chronic pancreatitis or other malabsorption disorder.
Central Contact Person:
First Name & Middle Initial & Last Name or Official Title & Degree
Dana Leach, DNP
Phone
352-273-8933
Email
leachdd@medicine.ufl.edu
First Name & Middle Initial & Last Name or Official Title & Degree
Sarah Long, RN
Phone
352-273-8933
Email
sarah.long@medicine.ufl.edu
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Carl Pepine, MD
Organizational Affiliation
University of Florida
Official's Role
Principal Investigator
Facility Information:
Facility Name
UF Health Cardiovascular Clinic
City
Gainesville
State/Province
Florida
ZIP/Postal Code
32610
Country
United States
12. IPD Sharing Statement
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Study 3: Minocycline Decreases Microglia Activation
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