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The Neural Mechanisms of Anesthesia and Human Consciousness (Part 6)

Primary Purpose

Anesthesia, Unconsciousness

Status
Completed
Phase
Phase 4
Locations
Finland
Study Type
Interventional
Intervention
Dexmedetomidine
Propofol
S-ketamine
Sevoflurane
Placebo
Sponsored by
University of Turku
About
Eligibility
Locations
Arms
Outcomes
Full info

About this trial

This is an interventional basic science trial for Anesthesia

Eligibility Criteria

18 Years - 30 Years (Adult)MaleAccepts Healthy Volunteers

Inclusion Criteria:

  1. Male
  2. Age 18-30 years
  3. Good general health i.e. American Society of Anesthesiologists (ASA) physical status I
  4. Fluent in Finnish language
  5. Right handedness
  6. Written informed consent
  7. Good sleep quality

Exclusion Criteria:

  1. Chronic medication
  2. History of alcohol and/or drug abuse
  3. Strong susceptibility for allergic reactions
  4. Serious nausea in connection with previous anesthesia
  5. Strong susceptibility for nausea
  6. Any use of drugs or alcohol during the 48 hours preceding anesthesia
  7. Use of caffeine products 10-12 hours prior the study
  8. Smoking
  9. Clinically significant previous cardiac arrhythmia / cardiac conduction impairment
  10. Clinically significant abnormality in prestudy laboratory tests
  11. Positive result in the drug screening test
  12. Blood donation within 90 days prior to the study
  13. Participation in any medical study with an experimental drug or device during the preceding 60 days
  14. The study subject has undergone a prior PET or SPECT study
  15. Any contraindication to magnetic resonance imaging (MRI)
  16. Hearing impairment
  17. Detected unsuitability based on MRI scanning results if available before the PET scanning
  18. Sleep disorder or severe sleep problem

Sites / Locations

  • Turku PET Centre

Arms of the Study

Arm 1

Arm 2

Arm 3

Arm 4

Arm 5

Arm Type

Experimental

Experimental

Experimental

Experimental

Placebo Comparator

Arm Label

Dexmedetomidine

Propofol

S-ketamine

Sevoflurane

Placebo

Arm Description

Intravenous dexmedetomidine using target controlled infusion.

Intravenous propofol using target controlled infusion.

Intravenous S-ketamine using target controlled infusion.

Inhalational sevoflurane using target controlled inhalation.

Intravenous saline.

Outcomes

Primary Outcome Measures

Regional cerebral metabolism of glucose
Comparison of responsive and unresponsive subjects

Secondary Outcome Measures

EEG
64-channel EEG will be recorded and analyzed using time domain, spectral domain, functional connectivity, directed/effective connectivity and graph theoretical analysis methods.
Immunological effects
Blood samples will be draw at baseline (without drug), at the end of study drug administration and after PET scanning for the measurement of approximately 50 cytokines, chemokines and growth factors.
Metabolomic effects
Blood samples will be drawn at baseline (without drug), at the end of drug administration and after PET scanning for the measurement of more than 200 serum measures, including lipoprotein subclass distribution and lipoprotein particle concentration, low molecular weight metabolites, such as amino acids, 3-hydroxybutyrate and creatinine, and detailed molecular information on serum lipids, including free and esterified cholesterol, sphingomyelin and fatty acid saturation.
Gene expression
Blood samples will be collected at baseline (without drug), at the end of drug administration and after PET scanning for the measurement of RNA expression using whole genome microarray-based, massively parallel sequencing or quantitative reverse-transcription polymerase chain reaction based methods.
Psychological well-being
Psychological well-being and ill-being will be measured with a battery of scientifically validated scales just before initiating the study session and at the end of the study session.
Dream report
After terminating PET imaging, a structured interview is immediately conducted to verify a recollection or absence of recollection of subjective experiences during possible loss of responsiveness.

Full Information

First Posted
November 25, 2015
Last Updated
March 22, 2017
Sponsor
University of Turku
Collaborators
University of California, Irvine, University of Michigan, Yale University, University of Helsinki
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1. Study Identification

Unique Protocol Identification Number
NCT02624401
Brief Title
The Neural Mechanisms of Anesthesia and Human Consciousness (Part 6)
Official Title
The Neural Mechanisms of Anesthesia and Human Consciousness (Part 6)
Study Type
Interventional

2. Study Status

Record Verification Date
March 2017
Overall Recruitment Status
Completed
Study Start Date
January 2016 (undefined)
Primary Completion Date
March 13, 2017 (Actual)
Study Completion Date
March 13, 2017 (Actual)

3. Sponsor/Collaborators

Responsible Party, by Official Title
Principal Investigator
Name of the Sponsor
University of Turku
Collaborators
University of California, Irvine, University of Michigan, Yale University, University of Helsinki

4. Oversight

Data Monitoring Committee
No

5. Study Description

Brief Summary
Positron Emission Tomography (PET), Magnetic Resonance Imaging (MRI) and electroencephalography (EEG) studies will be carried out to reveal the neural correlates of consciousness. Consciousness of the subjects will be manipulated with anesthetic agents dexmedetomidine, propofol, S-ketamine and sevoflurane. One-hundred-and-sixty (160) healthy male subjects will be recruited to receive EC50 concentration of the anesthetic (40 dexmedetomidine, 40 propofol, 20 S-ketamine, 40 sevoflurane) or placebo (20) while being imaged for cerebral metabolic rate of glucose (CMRglu). Also genetic, immunological and metabolomics samples will be taken and analysed to find possible genetic factors explaining the variability in drug response and to find chemical fingerprints of acute drug effect.
Detailed Description
The explanation of consciousness poses one of the greatest challenges to science and philosophy in the 21st century. It remains unclear what consciousness is and how it emerges from brain activity. Positron Emission Tomography (PET), Magnetic Resonance Imaging (MRI) and electroencephalography (EEG) studies will be carried out to reveal the neural correlates of consciousness. Consciousness of the subjects will be manipulated with anesthetic agents dexmedetomidine acting through α2-agonism, with propofol and sevoflurane both mainly acting through the enhancement of gamma-aminobutyric acid (GABA) system, and with S-ketamine acting through N-methyl-D-aspartate (NMDA) receptor antagonism. One-hundred-and-sixty (160) healthy male subjects will be recruited to receive EC50 concentration of either dexmedetomidine, propofol, S-ketamine or sevoflurane, or placebo while being imaged for cerebral metabolic rate of glucose (CMRglu). 40 subjects will receive dexmedetomidine, 40 subjects propofol, 20 subjects S-ketamine, 40 subjects sevoflurane and 20 subjects will receive placebo. Also genetic, immunological and metabolomics samples will be taken and analysed to find possible genetic factors explaining the variability in drug response and to find possible immunological and chemical fingerprints of acute drug effect.

6. Conditions and Keywords

Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Anesthesia, Unconsciousness

7. Study Design

Primary Purpose
Basic Science
Study Phase
Phase 4
Interventional Study Model
Parallel Assignment
Masking
None (Open Label)
Allocation
Randomized
Enrollment
160 (Actual)

8. Arms, Groups, and Interventions

Arm Title
Dexmedetomidine
Arm Type
Experimental
Arm Description
Intravenous dexmedetomidine using target controlled infusion.
Arm Title
Propofol
Arm Type
Experimental
Arm Description
Intravenous propofol using target controlled infusion.
Arm Title
S-ketamine
Arm Type
Experimental
Arm Description
Intravenous S-ketamine using target controlled infusion.
Arm Title
Sevoflurane
Arm Type
Experimental
Arm Description
Inhalational sevoflurane using target controlled inhalation.
Arm Title
Placebo
Arm Type
Placebo Comparator
Arm Description
Intravenous saline.
Intervention Type
Drug
Intervention Name(s)
Dexmedetomidine
Other Intervention Name(s)
Dexdor
Intervention Description
Intravenous infusion
Intervention Type
Drug
Intervention Name(s)
Propofol
Other Intervention Name(s)
Propofol-Lipuro
Intervention Description
Intravenous infusion
Intervention Type
Drug
Intervention Name(s)
S-ketamine
Other Intervention Name(s)
Ketanest-S
Intervention Description
Intravenous infusion
Intervention Type
Drug
Intervention Name(s)
Sevoflurane
Other Intervention Name(s)
Sevorane
Intervention Description
Inhalation
Intervention Type
Drug
Intervention Name(s)
Placebo
Intervention Description
Intravenous infusion of saline (Ringer's Acetate)
Primary Outcome Measure Information:
Title
Regional cerebral metabolism of glucose
Description
Comparison of responsive and unresponsive subjects
Time Frame
40 min
Secondary Outcome Measure Information:
Title
EEG
Description
64-channel EEG will be recorded and analyzed using time domain, spectral domain, functional connectivity, directed/effective connectivity and graph theoretical analysis methods.
Time Frame
1 hour
Title
Immunological effects
Description
Blood samples will be draw at baseline (without drug), at the end of study drug administration and after PET scanning for the measurement of approximately 50 cytokines, chemokines and growth factors.
Time Frame
2 hours
Title
Metabolomic effects
Description
Blood samples will be drawn at baseline (without drug), at the end of drug administration and after PET scanning for the measurement of more than 200 serum measures, including lipoprotein subclass distribution and lipoprotein particle concentration, low molecular weight metabolites, such as amino acids, 3-hydroxybutyrate and creatinine, and detailed molecular information on serum lipids, including free and esterified cholesterol, sphingomyelin and fatty acid saturation.
Time Frame
2 hours
Title
Gene expression
Description
Blood samples will be collected at baseline (without drug), at the end of drug administration and after PET scanning for the measurement of RNA expression using whole genome microarray-based, massively parallel sequencing or quantitative reverse-transcription polymerase chain reaction based methods.
Time Frame
2 hours
Title
Psychological well-being
Description
Psychological well-being and ill-being will be measured with a battery of scientifically validated scales just before initiating the study session and at the end of the study session.
Time Frame
2 hours
Title
Dream report
Description
After terminating PET imaging, a structured interview is immediately conducted to verify a recollection or absence of recollection of subjective experiences during possible loss of responsiveness.
Time Frame
1 hour
Other Pre-specified Outcome Measures:
Title
Drug concentration in plasma or end-tidal
Time Frame
1 hour

10. Eligibility

Sex
Male
Minimum Age & Unit of Time
18 Years
Maximum Age & Unit of Time
30 Years
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Eligibility Criteria
Inclusion Criteria: Male Age 18-30 years Good general health i.e. American Society of Anesthesiologists (ASA) physical status I Fluent in Finnish language Right handedness Written informed consent Good sleep quality Exclusion Criteria: Chronic medication History of alcohol and/or drug abuse Strong susceptibility for allergic reactions Serious nausea in connection with previous anesthesia Strong susceptibility for nausea Any use of drugs or alcohol during the 48 hours preceding anesthesia Use of caffeine products 10-12 hours prior the study Smoking Clinically significant previous cardiac arrhythmia / cardiac conduction impairment Clinically significant abnormality in prestudy laboratory tests Positive result in the drug screening test Blood donation within 90 days prior to the study Participation in any medical study with an experimental drug or device during the preceding 60 days The study subject has undergone a prior PET or SPECT study Any contraindication to magnetic resonance imaging (MRI) Hearing impairment Detected unsuitability based on MRI scanning results if available before the PET scanning Sleep disorder or severe sleep problem
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Harry Scheinin, MD
Organizational Affiliation
Turku PET Centre, University of Turku, Turku, Finland
Official's Role
Principal Investigator
Facility Information:
Facility Name
Turku PET Centre
City
Turku
ZIP/Postal Code
FI-20521
Country
Finland

12. IPD Sharing Statement

Plan to Share IPD
No
Citations:
PubMed Identifier
22492049
Citation
Langsjo JW, Alkire MT, Kaskinoro K, Hayama H, Maksimow A, Kaisti KK, Aalto S, Aantaa R, Jaaskelainen SK, Revonsuo A, Scheinin H. Returning from oblivion: imaging the neural core of consciousness. J Neurosci. 2012 Apr 4;32(14):4935-43. doi: 10.1523/JNEUROSCI.4962-11.2012.
Results Reference
background
PubMed Identifier
24025060
Citation
Langsjo JW, Revonsuo A, Scheinin H. Harnessing anesthesia and brain imaging for the study of human consciousness. Curr Pharm Des. 2014;20(26):4211-24.
Results Reference
background
PubMed Identifier
34534172
Citation
Nummela AJ, Laaksonen LT, Laitio TT, Kallionpaa RE, Langsjo JW, Scheinin JM, Vahlberg TJ, Koskela HT, Aittomaki V, Valli KJ, Revonsuo A, Niemi M, Perola M, Scheinin H. Effects of dexmedetomidine, propofol, sevoflurane and S-ketamine on the human metabolome: A randomised trial using nuclear magnetic resonance spectroscopy. Eur J Anaesthesiol. 2022 Jun 1;39(6):521-532. doi: 10.1097/EJA.0000000000001591. Epub 2021 Sep 22.
Results Reference
derived
PubMed Identifier
29935583
Citation
Laaksonen L, Kallioinen M, Langsjo J, Laitio T, Scheinin A, Scheinin J, Kaisti K, Maksimow A, Kallionpaa RE, Rajala V, Johansson J, Kantonen O, Nyman M, Siren S, Valli K, Revonsuo A, Solin O, Vahlberg T, Alkire M, Scheinin H. Comparative effects of dexmedetomidine, propofol, sevoflurane, and S-ketamine on regional cerebral glucose metabolism in humans: a positron emission tomography study. Br J Anaesth. 2018 Jul;121(1):281-290. doi: 10.1016/j.bja.2018.04.008. Epub 2018 May 8.
Results Reference
derived

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The Neural Mechanisms of Anesthesia and Human Consciousness (Part 6)

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