High-fat Overfeeding, Hepatokines and Appetite Regulation (OVEREAT)
Insulin Resistance, Type2 Diabetes Mellitus, Non-Alcoholic Fatty Liver Disease
About this trial
This is an interventional basic science trial for Insulin Resistance focused on measuring Hepatokines, High Fat Diet, Glycaemic Control, Fibroblast Growth Factor 21, Leukocyte Cell-derived Chemotaxin 2, Fetuin-A, Appetite, Ghrelin, Peptide YY
Eligibility Criteria
Inclusion Criteria:
- Recreationally active - ≤ 2 structured exercise sessions per week
- BMI between 18.5 - 27.9 kg/m2
- Body fat percentage < 20%
- Metabolically healthy - No known cardiovascular or metabolic disease such as diabetes, respiratory or heart disease.
- Non-smoker
- Weight stable in the past 6 months
- Normal fasting blood glucose levels (3.6 - 5.5 mmol/l)
Exclusion Criteria:
- Contraindications to exercise
- Needle Phobia
Sites / Locations
- National Centre for Sport and Exercise Medicine, Loughborough University
- Clifton Campus, Nottingham Trent University
Arms of the Study
Arm 1
Arm 2
Experimental
No Intervention
High-fat diet
Control diet
Participants will consume a hypercaloric, high-fat diet. Participants will be provided with all the food during the week and will be instructed to consume all of the foods provided and no extra calorie containing food or drink. In the event of leftover food, participants will be asked to return the food for measurement and subsequent subtraction from their total energy intake.
Participants will consume their normal 'habitual' diet for seven days which will be compared to their habitual diet recorded by a three day food diary before commencing the two diets. Participants will be instructed to carry on as normal and eat their usual diet and this period will be used as a comparator to the high-fat diet. They will also be told to record their food intake for 3 days during the diet to quantify their control diet.