Impact of Fish Oil Dose on Tissue Content and Function
Aging, Sarcopenia, Muscle Atrophy
About this trial
This is an interventional basic science trial for Aging focused on measuring Omega-3 polyunsaturated fatty acids, Fish oil supplementation, Washout, Dosing strategy
Eligibility Criteria
Inclusion Criteria:
- Provide valid informed consent prior to any study procedure
- Males and females 18-35 years of age or 60+ years of age
- BMI between 18-29 kg/m2
- Free of musculoskeletal injuries
- Willing to avoid alcohol in the 48-h period prior to the visits
- Willing to sustain their current diet and lifestyle and not to make conscious changes for the duration of the study
- An omega-3 status of less than 20% seen in whole blood taken during the screening visit.
- Willing to sustain current use of supplementation/anti-depressants or other medication not interfering with the study results.
- Women: not currently pregnant, not intending to become pregnant in the coming 5 months or lactating.
- Women: willing to maintain current use of contraceptives or post-menopausal supplementation if any for the duration of the study.
- Not allergic to fish, shellfish, seaweed, iodine, anesthetics, nickel or chrome.
Exclusion Criteria:
- Smoker
- Adherence to a strict vegan/vegetarian diet
- Treatment for cardiovascular diseases or blood pressure >140/90 mmHg
- Any diseases or medication that cause fat malabsorption (intestine issues such as celiac disease, Crohn's disease,chronic pancreatitis, or cystic fibrosis; liver and biliary disease, diarrhoea, steatorrhea)
- Diabetes or other (metabolic) disease that induce muscle wasting
- Surgery in prior 6 months
- Currently being on FO supplementation
- Current participation in another clinical trial, or in a trial within the past month
- For women: pregnant, intention to get pregnant during the course of the study or lactating
Sites / Locations
- University of Stirling
Arms of the Study
Arm 1
Arm 2
Arm 3
Arm 4
Experimental
Experimental
Experimental
Experimental
Young loading dose group
Old loading dose group
Young constant dose group
Old constant dose group
Participants (18-35) will receive a loading dose of fish oil supplementation during the first 4 weeks of the intervention period of the study. In the last 8 weeks participants will receive a maintenance dose of fish oil supplementation. The total amount of EPA/DHA received throughout the supplementation period will be the same as the old group.
Participants (60y+) will receive a loading dose of fish oil supplementation during the first 4 weeks of the intervention period of the study. In the last 8 weeks participants will receive a maintenance dose of fish oil supplementation. The total amount of EPA/DHA received throughout the supplementation period will be the same as the young group.
Participants (18-35y) will receive a constant dose of fish oil supplementation throughout the intervention period of the study. Total amount of EPA/DHA received throughout the 12 weeks supplementation period will be the same as the loading groups.
Participants (60y+) will receive a constant dose of fish oil supplementation throughout the intervention period of the study. Total amount of EPA/DHA received throughout the 12 weeks supplementation period will be the same as the loading groups.