Body-weight
Measured with calibrated scale in kg
Height
Measured with wall-mounted stadiometer in cm
Body Mass Index
Calculated by weight (kg) divided to height (m2)
Body weight composition
Measured by bioimpedance (Tanita SC 330-S)
Waist circumference
Steel measuring tape at the umbilicus in cm.
Sarcopenia assessment_skeletal muscle strength
grip strength, kg: Handheld dynamometer and the maximum value from either hand will be analyzed (Jamar dynamometer; Sammons Preston Rolyan, Bolingbrook, IL)
Sarcopenia assessment_muscle mass
Muscle mass, wasting and turnover: assessed by Magnetic Nuclear Resonance (MNR) measured lumbar 3rd vertebra
Sarcopenia assessment_muscle physical performance
Gait speed, m/s: length of the walking course divided by the time
Compliance of physical activity recommendations
IPAQ-E Spanish version
Compliance of the dietary recommendations
Dietary record: 3-day dietary record
Dietary compliance markers
Folin-Ciocalteau method adjusted by creatinine values, measured in 24h urine samples
Specific phenolic compounds_dietary compliance markers
Specific phenolic compounds and metabolites assessed by Ultra performance liquid chromatography - tandem mass spectrometer (UPLC-MS/MS) in urine samples
Phenolic metabolites_dietary compliance markers
Phenolic metabolites by UPLC-MS/MS in plasma
Sarcopenia and Quality of life
SarQoL® test assessed by quality of life test adapted to sarcopenic subjects. It is scored from 0 to 100, where higher values indicate better quality of life.
Fasting blood glucose (FBG)
by standardized methods in an automated analyzer (Beckman Coulter-Synchron, Galway, Ireland) in (mmol/L)
Insulin
by standardized methods in an automated analyzer (Beckman Coulter-Synchron, Galway, Ireland) in (IU/mL)
Homeostasis model assessment index (HOMA index)
Calculated by fasting insulin (μUI/mL) x fasting glucose (mmol/L) / 22,5
Lipid profile
Total cholesterol (TC), high density lipoprotein cholesterol (HDLc), low density lipoprotein cholesterol (LDLc) and Total triglycerides (TG) in mmol/L by standardized enzymatic automated methods in an autoanalyzer (Beckman Coulter-Synchron, Galway, Ireland)
Apolipoproteins
Apolipoprotein A1 (ApoA1) and apolipoprotein B100 (ApoB100) in mg/dL by standardized enzymatic automated methods in an autoanalyzer (Beckman Coulter-Synchron, Galway, Ireland)
Apolipoproteins ratio
Calculated by ApoA1 divided to ApoB100
Vascular parameters_endothelial function
Ischemic reactive hyperemia (IRH) with Laser-Doppler Linear Periflux 5000 (Perimed AB, Järfälla, Stockholm, Sweden)
Vascular parameters_blood pressure
Systolic blood pressure (SBP) and Diastolic blood pressure (DBP) in mm Hg by automatic sphygmomanometer (OMRON HEM-907; Peroxfarma, Barcelona, Spain).
Vascular parameters_pulse pressure
Calculated by SBP - DBP
Endothelin
Endothelin in pg/mL assessed by Commercial ELISA
Endothelial dysfunction markers
E-selectin (CD62E), P-selectin (CD62P), Intercellular Cell Adhesion Molecule 1 (ICAM-1 (CD54)) and Vascular Cell Adhesion Molecule 1 (VCAM-1 (CD106) assessed by multi-analyte ELISArray kits.
Oxidative biomarkers_Oxidized LDL
Oxidized LDL (oxLDL) assessed by commercial ELISA.
Oxidative biomarkers_Superoxide Dismutase
Superoxide Dismutase (SOD) in U/g Hb assessed by enzymatic assay
Oxidative biomarkers_Glutathione peroxidase
Glutathione peroxidase (GSHPx) in nmol/mL assessed by enzymatic assay
Inflammation biomarkers_High sensitivity C-reactive protein
High sensitivity C-reactive protein (hsCRP) in mg/L assessed by Immunoturbidimetry on an autoanalyzer (Roche Diagnostics Systems, Madrid, Spain)
Inflammation biomarkers_interleukine-6
Interleukine-6 (IL-6) in pg/mL assessed by Immunoturbidimetry on an autoanalyzer (Roche Diagnostics Systems, Madrid, Spain)
Inflammation biomarkers_uric acid
Uric acid in mg/dL is assessed by Immunoturbidimetry on an autoanalyzer (Roche Diagnostics Systems, Madrid, Spain)
Inflammation biomarkers_tumour necrosis factor-α
Tumour necrosis factor-α (TNF-α) in pg/mL is assessed by commercial ELISA kit.
Renal function_creatinine
Creatinine in mg/dL is assessed by standardized methods in a Cobas Mira Plus autoanalyzer (Roche Diagnostics Systems, Madrid, Spain) in 24 h urine samples
Renal function_glomerular filtration rate
Glomerular filtration rate (GFR) in mL/minutes/1.73m2 is assessed by equation Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI).
Endocrine system
Testosterone (pg/mL) and growth hormone (GH) (pg/mL)) assessed by in a Cobas Mira Plus autoanalyzer (Roche Diagnostics Systems, Madrid, Spain)
Proteome profiling
Whole proteome assessed by nano Liquid chromatography-mass spectrometry (nLC-MS) according to our previous work.
Gut microbiota_phyla composition and functionality
Phyla composition and functionality assessed by whole genomic content sequencing. Illumina platform will be used to obtain the metagenomics and metatranscriptomics of each sample following previous protocols developed in Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO-Salud Pública (Valencia)).
Gut microbiota_short chain fatty acids
Short chain fatty acids (SCFA): bile acids, sterols, buthyrate, and branched chain amino acids and sterols assessed by gas chromatography (GC)