Inflammatory Status of Visceral Adipose Tissue in Obese Patients Related to Periodontitis Severity (BACTERIOB)

Comparative Evaluation of the Inflammatory Status of Visceral Adipose Tissue From Obese Patients According to the Presence and Severity of Periodontitis

The research aims to compare the inflammatory status of visceral adipose tissue from obese patients depending on the presence and severity of periodontitis, in order to establish a potential causal link between the severity of the bacterial contamination and insulin resistance.

Recruitment of patients will be achieved in the department of digestive surgery. It will involve 122 patients with severe obesity undergoing bariatric surgery scheduled within the digestive surgery department. Patients recruited will be examined for evaluating the presence and severity of periodontitis, and separated into either group 1: for patients with moderate or severe periodontitis, and or group 2: for patients without or with mild periodontitis. The day before the surgery, blood sample will be collected for the analysis of circulating metabolic and inflammatory markers. Collection of stimulated saliva, periodontal sample and faeces samples (50 g) will also be carried out for bacteria determination. During the surgical procedure, a sample of visceral adipose tissue (50 g) will be collected and used for periodontal bacteria detection and inflammatory markers analyses.

Periodontal examination Biological samples collection: periodontal, saliva, blood, fecal, visceral adipose tissue

Periodontal examination Biological samples collection: periodontal, saliva, blood, fecal, visceral adipose tissue

Inflammatory status of visceral adipose tissue from obese patients depending on the presence and severity of periodontitis

Measurements of major adipocytokines (Interleukine 6, Leptin, Adiponectin, Tumor Necrosis Factor-alpha, Monocyte Chemoattractant Protein-1, macrophage F4/80 receptor, CD11c, nuclear factor-kappa B, Toll Like Receptor 2/4 )

Aggregatibacter actinomycetemcomitans, Fusibacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola and Tannerella forsythia

Link between adipose tissue inflammatory status, bacterial contamination and metabolic profile of patients

adipose tissue inflammatory status, periodontal bacterial contamination,diabetic disorders, lipid metabolic profile of patients

Link between the presence of periodontal pathogens in adipose tissue and their detection in saliva and plasma

Inclusion Criteria: Patient eligible for bariatric surgery scheduled within the digestive surgery Patient affiliated to Social Security system or equivalent Exclusion Criteria: Treatment with steroids or anti-inflammatory drugs in progress (or stopped for less than one month) Patients who received antibiotics within 3 months prior to the bariatric surgery for whatever duration Dental current (or older than 3 months) Participation in another research protocol